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Raman spectroscopy monitors the changes in the oxygenation state of a single human red blood cell while it is placed under mechanical stress using optical tweezers.
Credits: ICFO
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Activities:
Biological systems function by traversing various mechanochemical pathways, thus correlating force measurements sensitive to detect forces down to 50 fN with the monitoring of chemical processes is an ideal method. Using optically trappable SERS probes brings Raman process efficiency to the same level as fluorescence. We study also a colloidal particle in a space- and time modulated potential energy landscape created by optical traps as a model of the noise –assisted phenomena in biophysics. We use Photonic Force Microscope as a spectrometer studying emission and nonlinear processes by measuring the momentum transfer of emitted photons to the probe.
Postdoc position available starting July 2010
Projects:
Electrophoresis in an optical trap
Noise-assisted effects in physics and biophysics studied by the technique of optical traps
Novel optical methods for selfassembled nanostructures
Photonic Force Microscope as a spectrometer
Raman imaging of human cells
Raman spectroscopy of single living cells
Combining raman spectroscopy and photonic force microscopy
Available Resources:
- CW lasers at 532, 633, 635, 785, 830, 850, 985, and 1064 nm
- Spectrometer Acton equipped with a Roper Scientific CCD Camera cooled to -80C
- Spectrometer Andor equipped with a CCD Camera cooled to -60C
- PCR (polymerase chain reaction) and gel electrophoresis for amplification of DNA
- Microscope live-cell chambers
- Position detectors
Collaborations:
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COST - European Cooperation in the field of Scientific and Technical Research (http://www.cost.esf.org).
- Departament de Fisica Fonamental, Facultat de Fisica, Universitat de Barcelona (Prof. M. Rubi, Prof. F. Ritort).
- Unitat de Biofísica i Bioenginyeria, Departament de Ciències Fisiològiques-I, Facultat de Medicina, Universitat de Barcelona (Prof. D. Navajas).
- Universitat Autònoma de Barcelona, Department Genètica i de Microbiologia (Prof. M. Llagostera, Dr. S. Campoy).
- Institut de Ciencia de Materials de Barcelona (ICMAB-CSIC), Bellaterra, Spain (Prof. J. Veciana).
- Barcelona Supercomputer Centre (Prof. V. Guallar)
- Photonic4life – European network of excellence for biophysics (www.photonics4life.eu)
- Molecular Oncology Centre , Institut d´Investigació Biomèdica de Bellvitge (IDIBELL)
Barcelona (Prof. Angels Sierra Jiménez)
Links:
Group homepage
Contact:
 dmitri.petrov@icfo.es
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