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Raman spectroscopy monitors the changes in the oxygenation state of a single human red blood cell while it is placed under mechanical stress using optical tweezers.
Credits: ICFO
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Areas:
Biophotonics
Group associations:
Optical tweezers
Abstract:
Two benefits have been realized in the field of Raman spectroscopy and Photonic Force Microscopy: the ability to fixate a single micron sized object has allowed for the advent of single particle studies and the stability of an optical trap provides a system allowing a trapped particle to become a probe sensitive to measure forces down to 50 fN. The goal of this effort is to combine the two techniques within one system. Thus, correlating mechanical forces measurements with monitoring of structural information and chemical processes is an ideal method for revealing new insights that each technique alone would not be able to provide.
Funding:
MEC, Spain Grant No. NAN2004-09348-C04-02 and FIS2005- 02129
Collaborations:
1. Barcelona Supercomputer Center (ICREA Professor V. Guallar)
2. Universitat Autònoma de Barcelona, Departament de Genètica i de Microbiología (Prof. M. Llagostera, Dr. S. Campoy)
People in this project:
Dmitri Petrov
Saurabh Raj
Stefan Balint
Selected publications in this project:
Polarization Raman study of protein ordering by controllable RBC deformation, S. Rao, S. Bálint, L. C. Frias, D. Petrov, J. Raman Spectroc. [Online publication] (2009)
Raman study of mechanically induced oxygenation state transition of red blood cells using optical tweezers, S. Rao, S. Bálint, B. Cossins, V. Guallar, D. Petrov, Biophys. J. 96, 209-216 (2009)
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