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An optically trapped sphere partially covered by metal above a living cell.
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Areas:
Biophotonics
Group associations:
Optical tweezers
Abstract:
We combine Raman spectroscopy and Photonic Force Microscopy to get time- and spatial resolved information on biochemical and biophysical processes in single living cells and long molecules. Our work ranges from simpler systems such as red blood cells and DNA to those that are more complex such as brain tumor cells. To enhance the Raman efficiency to the same level as fluorescence we use optically trappable SERS probes that are produced by attaching silver colloids to the surfaces of micron sized dielectric spheres. The SERS probes are used to provide fast Raman imaging of cells and the injection of these probes in to cell systems allows for monitoring of drug diffusion.
Funding:
MEC, Spain Grant No. NAN2004-09348-C04-02 and FIS2005- 02129
Collaborations:
1. Hospital Vall d´Hebron (Barcelona)
Dr. David García-Dorado García
2. Department of Neurosciences
Institut for Biomedical Research August Pi Sunyer (IDIBAPS)
Hospital Clinic of Barcelona
Dr. Pablo Villoslada
3. Molecular Oncology Centre
Institut d´Investigació Biomèdica de Bellvitge (IDIBELL)
Barcelona
Dr. Angels Sierra Jiménez
4. Department of Biophysics, University of Pavol Jozef ˇSaf´arik, Koˇsice 04154,
Slovak Republic
People in this project:
Dmitri Petrov
Mónica Marro Sánchez
Saurabh Raj
Stefan Balint
Selected publications in this project:
Raman spectroscopy of optically trapped particles, D. V. Petrov, J. Opt. A: Pure Appl. Opt. 9, S139-S156 (2007)
The lag phase and G1 phase of a single yeast cell monitored by Raman microspectroscopy, G.P. Singh, G. Volpe, C. M. Creely, H. Grötsch, I. M. Geli, D. Petrov, J. Raman Spectrosc. 37, 858-864 (2006)
Raman spectroscopy on floating cells, C. Creely, G. P. Singh, G. Volpe, D.V. Petrov , Opt. Photon. News 16, 20 (2005)
Raman imaging of floating cells, C. Creely, G. Volpe, G. P. Singh, M. Soler, D.V. Petrov , Opt. Express 12, 6105-6110 (2005)
Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy, G. P. Singh, C. Creely, G. Volpe, H. Groetsch, D.V. Petrov
, Anal. Chem. 77, 2564-2568 (2005)
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