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Areas:
Biophotonics
Group associations:
Optical tweezers
Abstract:
Raman spectroscopy has been successfully applied to extract time resolved spectroscopic information from live cells in suspension and space-resolved information from live cells growing on a substrate. However, in order to extract space-resolved information from cells in suspension a new technique is required.
LINK BELOW:
This movie illustrates the problem of rotation with a single beam optical trap.
The new setup uses an SLM to allow the modulation of a tweezing beam such that multiple, controllable points are produced at the focal plane in order to trap and move a particle in suspension. These Holographic Optical Tweezers (HOT) can move and scan the cell with respect to a laser beam focused in the sample plane which is used to excite the Raman spectra. In this way an image can be created of a floating cell. This allows an extra dimension of information to be collected on cells whose natural environment is in suspension, e.g. blood cells.
LINK: Movie
Additional Information
Collaborations:
Dr. Timothy M. Thomson and Marta Soler, Cell Signalling and Cancer Group, Departamento de la Biología Molecular y Celular, Instituto de Biología Molecular, CSIC, Barcelona
People in this project:
Dmitri Petrov
Stefan Balint
Selected publications in this project:
Raman imaging of neoplastic cells in suspension, C. M. Creely, S. Mercadal, G. Volpe, M. Soler, D. V. Petrov, Proc. SPIE 6326, 63260U-1-8 (2006)
Raman spectroscopy on floating cells, C. Creely, G. P. Singh, G. Volpe, D.V. Petrov , Opt. Photon. News 16, 20 (2005)
Raman imaging of floating cells, C. Creely, G. Volpe, G. P. Singh, M. Soler, D.V. Petrov , Opt. Express 12, 6105-6110 (2005)
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